MYO9B Gene Polymorphism (SNP rs2305767) Associated with Celiac Disease in Iraqi Patients

Sardar  Khudhur; Özcan  Özkan; Jaleel  Samanje; Omar A Mahmoud

doi:10.47419/bjbabs.v7i1.439

Authors

Dr. Sardar Khudhur Ministry of Health, Baghdad, Iraq https://orcid.org/0009-0000-3443-4577
Dr. Özcan Özkan Department of Biology, Faculty of Science, Cankiri Karatekin University, Cankiri, Turkey https://orcid.org/0000-0002-5216-8361
Dr. Jaleel Samanje Colleague of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq https://orcid.org/0000-0001-9747-8120
Omar A Mahmoud Department of forensic biology, Higher institute of forensic sciences, Al- Nahrain University, Baghdad, Iraq https://orcid.org/0009-0005-6884-9398

DOI:

https://doi.org/10.47419/bjbabs.v7i1.439

Keywords:

Celiac disease, Myosin IXB gene, SNP, EMA, TTg antibody.

Abstract

Introduction: The MYO9B gene is involved in maintaining the intestinal barrier and is linked with a higher risk of developing celiac disease (CD). The aim of this investigation was to link the MYO9B gene polymorphism to CD in a patient sample from Iraq. Patients and methods: In Bagdad, Iraq, 30 CD patients and 20 control individuals participated in the study. The MYO9B gene polymorphism was analyzed by gene sequencing. Enzyme-linked immunosorbent serologic assay was used to determine the concentrations of anti-transglutaminase (tTG) Ab, anti-gliadin Ab, and anti-endomysial (EMA) Ab in CD patients’ serum.
Results: Three genotypes (Homozygote CC, Heterozygote CT, and Mutant Homozygote TT) and two alleles (C and T) were found for single nucleotide polymorphism (SNP) rs2305767. CD patients had three genotypes (CC, CT, and TT) for SNP, but only CC genotype was discovered in controls. The Hardy–Weinberg equilibrium analysis showed a significant difference between the patients and controls. Patients with CD had greater serum levels of gliadin, EMA, and tTG than healthy controls (P < 0.05).
Conclusion: Our findings suggest that SNP rs2305767 was associated with CD.