Diagnostic methods and protocols used in investigating Toxoplasma gondii in humans: A review

Authors

  • Shmeeran S. Ismael Department of Medical Laboraatories Sciences, College of Health Sciences, University of Duhok, Duhok, Iraq

DOI:

https://doi.org/10.47419/bjbabs.v2i04.72

Keywords:

serological diagnosis, serotyping, T. gondii, toxoplasmosis, molecular diagnosis

Abstract

Toxoplasmosis, a zoonotic disease, is a parasitic infection caused by a coccidian protozoan Toxoplasma gondii. In immunocompetent people, the infection is asymptomatic, while it can produce serious signs in immunocompromised people and in developing fetuses. Accurate diagnosis is dependent, mainly, on the clinical features. However, in immunocompromised patients, the diagnosis is very difficult and may lead to misdiagnosis and improper treatment. Today, molecular diagnosis and serotyping are widely used for the diagnosis of T. gondii in many countries. The aim of the present short review is to highlight the current diagnostic methods and protocols that are used for the diagnosis of T. gondii in humans.

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References

Ashburn, D., Chatterton, J. M., Evans, R., Joss, A. W. & Ho-Yen, D. O. (2001). Success in the toxoplasma dye test. The Journal of infection, 42(1), 16–19. https://doi.org/10.1053/jinf.2000.0764 DOI: https://doi.org/10.1053/jinf.2000.0764

Augusto, L., Amin, P. H., Wek, R. C. & Sullivan, W. J., Jr (2019). Regulation of arginine transport by GCN2 eIF2 kinase is important for replication of the intracellular parasite Toxoplasma gondii. PLoS pathogens, 15(6), e1007746. https://doi.org/10.1371/journal.ppat.1007746 DOI: https://doi.org/10.1371/journal.ppat.1007746

Bastien, P., Jumas-Bilak, E., Varlet-Marie, E. and Marty, P. (2007). Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France. Clinical Microbiology and Infection, 13: 430–433. DOI: https://doi.org/10.1111/j.1469-0691.2006.01642.x

Berrouch, S., Escotte-Binet, S., Harrak, R., Huguenin, A., Flori, P., Favennec, L., Villena, I. and Hafid, J. (2020). Detection methods and prevalence of transmission stages of Toxoplasma gondii, Giardia duodenalis and Cryptosporidium spp. in fresh vegetables: a review. Parasitology, 147(5), 516–532. https://doi.org/10.1017/S0031182020000086 DOI: https://doi.org/10.1017/S0031182020000086

Bin Dajem, S. M. & Almushait, M. A. (2012). Detection of Toxoplasma gondii DNA by PCR in blood samples collected from pregnant Saudi women from the Aseer region, Saudi Arabia. Annals of Saudi medicine, 32(5), 507–512. https://doi.org/10.5144/0256-4947.2012.14.7.1200 DOI: https://doi.org/10.5144/0256-4947.2012.14.7.1200

Calderaro, A., Piccolo, G., Gorrini, C., Peruzzi, S., Zerbini, L., Bommezzadri, S., Dettori, G. and Chezzi, C. (2006). Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii. Acta biomedica : Atenei Parmensis, 77(2), 75–80

Chatterton, J. M., Evans, R., Ashburn, D., Joss, A. W. and Ho-Yen, D. O. (2002).Toxoplasma gondii in vitro culture for experimentation. J Microbiol Methods., 51:331–5 DOI: https://doi.org/10.1016/S0167-7012(02)00101-X

De Berardinis, A., Paludi, D., Pennisi, L. and Vergara, A. (2017). Toxoplasma gondii, a Foodborne Pathogen in the Swine Production Chain from a European Perspective. Foodborne pathogens and disease, 14(11), 637–648. https://doi.org/10.1089/fpd.2017.2305 DOI: https://doi.org/10.1089/fpd.2017.2305

Di Carlo, P., Casuccio, A., La Chiusa, S., Mazzola, A., Pampinella, D., Romano, A., Schimmenti, M. G., Titone, L. & Mancuso, G. (2007). Diagnosis of congenital toxoplasmosis: pre- and post-natal evaluation in Sicilian (Italy) epidemiological area. Preliminary data. Parassitologia, 49(1-2), 39–41.

Fuentes, I., Rubio, J. M., Ramírez, C. and Alvar, J. (2001). Genotypic characterization of Toxoplasma gondii strains associated with human toxoplasmosis in Spain: direct analysis from clinical samples. Journal of clinical microbiology, 39(4), 1566–1570. https://doi.org/10.1128/JCM.39.4.1566-1570.2001 DOI: https://doi.org/10.1128/JCM.39.4.1566-1570.2001

Furtado, J. M., Smith, J. R., Belfort, R., Jr, Gattey, D. & Winthrop, K. L. (2011). Toxoplasmosis: a global threat. Journal of global infectious diseases, 3(3), 281–284. https://doi.org/10.4103/0974-777X.83536 DOI: https://doi.org/10.4103/0974-777X.83536

Hecker, Y. P., Masson, F. M., Armendano, J. I., Cora, J., Olivares, C. F., Gual, I., Pardini, L., Moore, D. P., Moré, G. and Cantón, G. J. (2018). Evaluation of frequency of antibodies against Toxoplasma gondii, Neospora caninum and Sarcocystis spp. and transmission routes in sheep from Humid Pampa, Argentina. Acta parasitologica, 63(2), 416–421. https://doi.org/10.1515/ap-2018-0048 DOI: https://doi.org/10.1515/ap-2018-0048

Hill, D. and Dubey, J. P. (2002). Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect., 8: 634–640 DOI: https://doi.org/10.1046/j.1469-0691.2002.00485.x

Hou, Z. F., Su, S. J., Liu, D. D., Wang, L. L., Jia, C. L., Zhao, Z. X., Ma, Y. F., Li, Q. Q., Xu, J. J. and Tao, J. P. (2018). Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 60, 17–25. https://doi.org/10.1016/j.meegid.2018.02.007 DOI: https://doi.org/10.1016/j.meegid.2018.02.007

Mohammad, M., Ahmed, S. and Hussain, A. (2012). Seroprevalence of Toxoplasma gondii between couples in Ramadi city using enzyme linked immunosorben assay (ELISA). Int J Med Sci., 4:55-9 DOI: https://doi.org/10.5897/IJMMS11.101

Montoya J. G. (2002). Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. The Journal of infectious diseases, 185 Suppl 1, S73–S82. https://doi.org/10.1086/338827 DOI: https://doi.org/10.1086/338827

Montoya, J. G. and Remington, J. S. (1995). Studies on the serodiagnosis of toxoplasmic lymphadenitis. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 20(4), 781–789. https://doi.org/10.1093/clinids/20.4.781 DOI: https://doi.org/10.1093/clinids/20.4.781

Montoya, J.G. and Remington, J.S. (2000). Toxoplasma gondii. In: Mandell GL, Bennett JE, Dolin R, eds. Principles and practice of infectious diseases. Philadelphia: Churchill Livingstone, 2858–88

Moshfeghi, D. M., Dodds, E. M., Couto, C. A., Santos, C. I., Nicholson, D. H., Lowder, C. Y. & Davis, J. L. (2004). Diagnostic approaches to severe, atypical toxoplasmosis mimicking acute retinal necrosis. Ophthalmology, 111(4), 716–725. https://doi.org/10.1016/j.ophtha.2003.07.004 DOI: https://doi.org/10.1016/j.ophtha.2003.07.004

Remington, J. S., McLeod, R., Thulliez, P. and Desmonts, G. (2001). Toxoplasmosis. In: Remington JS, Klein J, eds. Infectious diseases of the fetus and newborn infant. 5th ed. Philadelphia: W.B. Saunders, 205–346

Robert-Gangneux, F., Sterkers, Y., Yera, H., Accoceberry, I., Menotti, J., Cassaing, S., Brenier-Pinchart, M. P., Hennequin, C., Delhaes, L., Bonhomme, J., Villena, I., Scherer, E., Dalle, F., Touafek, F., Filisetti, D., Varlet-Marie, E., Pelloux, H. and Bastien, P. (2015). Molecular diagnosis of toxoplasmosis in immunocompromised patients: a 3-year multicenter retrospective study. Journal of clinical microbiology, 53(5), 1677–1684. https://doi.org/10.1128/JCM.03282-14 DOI: https://doi.org/10.1128/JCM.03282-14

Rostami, A., Karanis, P. and Fallahi, S. (2018). Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection. Infection, 46(3), 303–315. https://doi.org/10.1007/s15010-017-1111-3 DOI: https://doi.org/10.1007/s15010-017-1111-3

Sousa, S., Canada, N., Correia da Costa, J. M. & Dardé, M. L. (2010). Serotyping of naturally Toxoplasma gondii infected meat-producing animals. Veterinary parasitology, 169(1-2), 24–28. https://doi.org/10.1016/j.vetpar.2009.12.025 DOI: https://doi.org/10.1016/j.vetpar.2009.12.025

Su, C., Shwab, E. K., Zhou, P., Zhu, X. Q. and Dubey, J. P. (2010). Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii. Parasitology, 137(1), 1–11. https://doi.org/10.1017/S0031182009991065 DOI: https://doi.org/10.1017/S0031182009991065

Tridapalli, E., Capretti, M., Farneti, G., Marangoni, A., Cevenini, R. and Faldella, G. (2008). Congenital toxoplasmosis: the importance of the western blot method to avoid unnecessary therapy in potentially infected newborns. Acta paediatrica (Oslo, Norway : 1992), 97(9), 1298–1300. https://doi.org/10.1111/j.1651-2227.2008.00905.x DOI: https://doi.org/10.1111/j.1651-2227.2008.00905.x

Verma, S. K., Knowles, S., Cerqueira-Cezar, C. K., Kwok, O.C., Jiang, T., Su, C. and Dubey, J. P. (2018). An update on Toxoplasma gondii infections in northern sea otters (Enhydra lutris kenyoni) from Washington State, USA. Vet Parasitol. https://doi.org/10.1016/j.vetpar.2018.05.011 DOI: https://doi.org/10.1016/j.vetpar.2018.05.011

Wang, X. L., Dong, L., Zhang, L., Lv, Y., Li, Q. and Li, H. (2018c). Seroprevalence and genetic characterization of Toxoplasma gondii in naturally-infected synanthropic rodents in Yunnan Province, Southwestern China. J Parasitol. https://doi.org/10.1645/17-156 DOI: https://doi.org/10.1645/17-156

Weiss, L. M. and Kim, K. (2007).Toxoplasma gondii: the model apicomplexan: perspectives and methods. Illustrated ed. Academic Press, 367–86

Xiao, J., Buka, S. L., Cannon, T. D., Suzuki, Y., Viscidi, R. P., Torrey, E. F. & Yolken, R. H. (2009). Serological pattern consistent with infection with type I Toxoplasma gondii in mothers and risk of psychosis among adult offspring. Microbes and infection, 11(13), 1011–1018. https://doi.org/10.1016/j.micinf.2009.07.007 DOI: https://doi.org/10.1016/j.micinf.2009.07.007

Published

02-11-2021

How to Cite

Ismael, S. (2021). Diagnostic methods and protocols used in investigating Toxoplasma gondii in humans: A review. Baghdad Journal of Biochemistry and Applied Biological Sciences, 2(04), 181–186. https://doi.org/10.47419/bjbabs.v2i04.72

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Narrative reviews

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