FOXA1 expression in Iraqi women with ER+ breast cancer

Authors

  • Iman Hatif Al-Bedairy Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq
  • Mais S. Shamsa College of Medicine, University of Kufa, Kufa, Iraq
  • Safaa uldeen Salem College of Medicine, University of Kufa, Kufa, Iraq
  • Mohammed Mahdi DNA Forensic Center for Research and Training, Al-Nahrain University, Baghdad, Iraq
  • Karam Dawood Department of Medical Laboratory Technology, Al-Esraa University College, Baghdad, Iraq
  • Abdul Hussein M. Al Faisal Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.47419/bjbabs.v2i02.43

Keywords:

breast cancer, ER , FOXA1, immunohistochemistry, RT-qPCR

Abstract

Background: Breast cancer (BC) is a heterogeneous disease that can be classified into many subtypes according to histopathological and molecular characteristics. Forkhead box protein A1 (FOXA1) is a pioneer factor of estrogen receptor (α-ER)-chromatin binding and function. FOXA1 expression is related to luminal BC with a good prognosis.

Objectives: The present study is sought to determine the FOXA1 expression in Iraqi women with ER+ BC from fresh tissue.

Methods: Forty-eight fresh malignant breast tissues were analyzed by immunohistochemistry assay to choose ER+ samples, and then by RT-qPCR to evaluate FOXA1 gene expression.

Results: The ER-positive samples were (72.91%) of the total samples, luminal A was the most common molecular subtype with (56.25%).

Conclusions: Highly significant FOXA1 expression was found in Iraqi women with BC makes it eligible to be a good predictor or a biomarker for BC.

 

PDF file of uncorrected proof will be updated soon.

Downloads

Download data is not yet available.

References

Anita Mangia et al. “Should Tumor Infiltrating Lymphocytes, Androgen Receptor, and FOXA1 Expression Predict the Clinical Outcome in Triple Negative Breast Cancer Patients?” Cancers (Basel) 11(9) (2019), pp. 1393–1393. DOI: 10.3390/cancers11091393.

Antoni Hurtado et al. “FOXA1 is a key determinant of estrogen receptor function and endocrine response”. Nat Genet 43(1) (2011), pp. 27–33. DOI: 10.1038/ng.730.

Azizun-Nisa et al. “Comparison of ER, PR and HER-2/neu (C-erb B 2) reactivity pattern with histologic grade, tumor size and lymph node status in breast cancer”. Asian Pac J Cancer Prev 9(4) (2008), pp. 553–556.

Bing Song et al. “Targeting FOXA1-mediated repression of TGF-β signaling suppresses castration-resistant prostate cancer progression”. J Clin Invest 129(2) (2018), pp. 569–582. DOI: 10.1172/jci122367.

Derek F. Amanatullah et al. “Local estrogen axis in the human bone microenvironment regulates estrogen receptor-positive breast cancer cells”. Breast Cancer Res 19(1) (2017), pp. 121–121. DOI: 10.1186/s13058-017-0910-x.

Esther Rheinbay et al. “Recurrent and functional regulatory mutations in breast cancer”. Nature 547(7661) (2017), pp. 55–60. DOI: 10.1038/nature22992.

G Jiang et al. “Cooperativity of co-factor NR2F2 with Pioneer Factors GATA3, FOXA1 in promoting ERα function”. Theranostics 9(22) (2019), pp. 6501–6516. DOI: 10.7150/thno.34874.

G M Clark, C K Osborne, and W L McGuire. “Correlations between estrogen receptor, progesterone receptor, and patient characteristics in human breast cancer.” J Clin Oncol 2(10) (1984), pp. 1102–1109. DOI: 10.1200/jco.1984.2.10.1102.

Gina M. Bernardo and Ruth A. Keri. “FOXA1: a transcription factor with parallel functions in development and cancer”. Biosci Rep 32(2) (2012), pp. 113–130. DOI: 10.1042/bsr20110046.

Huiyan Ma et al. “Body mass index at age 18 years and recent body mass index in relation to risk of breast cancer overall and ER/PR/HER2-defined subtypes in white women and African-American women: a pooled analysis”. Breast Cancer Res 20(1) (2018), pp. 5–6. DOI: 10.1186/s13058-017-0931-5.

J Laganière et al. “From the Cover: Location analysis of estrogen receptor alpha target promoters reveals that FOXA1 defines a domain of the estrogen response”. Proc Natl Acad Sci U S A 102 (2005), pp. 11651–11656. DOI: 10.1073/pnas.0505575102.

J Rosai. Rosai and Ackerman’s Surgical Pathology. Ed. by and others. 10th. Vol. 1. Elsevier Inc. Italy, 2011.

J Xu et al. “Prediction of tumor mutation burden in breast cancer based on the expression of ER, PR, HER -2 and Ki-67”. Onco Targets Ther 11 (2018), pp. 2269–2275.

Jason S. Carroll et al. “Chromosome-Wide Mapping of Estrogen Receptor Binding Reveals Long-Range Regulation Requiring the Forkhead Protein FoxA1”. Cell 122(1) (2005), pp. 33–43. DOI: 10.1016/j.cell.2005.05.008.

Jiafeng Shou et al. “Prognostic value of FOXA1 in breast cancer: A systematic review and meta-analysis”. Breast 27 (2016), pp. 35–43. DOI: 10.1016/j.breast.2016.02.009.

Jonathan G Moggs and George Orphanides. “Estrogen receptors: orchestrators of pleiotropic cellular responses”. EMBO Rep 2(9) (2001), pp. 775–781. DOI: 10.1093/embo-reports/kve185.

Jonna Frasor et al. “Profiling of Estrogen Up- and Down-Regulated Gene Expression in Human Breast Cancer Cells: Insights into Gene Networks and Pathways Underlying Estrogenic Control of Proliferation and Cell Phenotype”. Endocrinology 144(10) (2003), pp. 4562–4574. DOI: 10.1210/en.2003-0567.

K Michailidou. “Association analysis identifies 65 new breast cancer risk loci”. Nature 551(7678) (2018), pp. 92–94. DOI: 10.1038/nature24284.

K Wang et al. “Clinical significance and prognostic value of Forkhead box A1 expression in human epithelial ovarian cancer”. Oncol Lett 15 (2018), pp. 4457–4462. DOI: 10.3892/ol.2018.7899.

KELI HE et al. “Clinicopathological significance of forkhead box protein A1 in breast cancer: A meta-analysis”. Exper Ther Med 11(6) (2016), pp. 2525–2530. DOI: 10.3892/etm.2016.3229.

L A Cirillo et al. “Binding of the winged-helix transcription factor HNF3 to a linker histone site on the nucleosome”. EMBO J 17(1) (1998), pp. 244–254. DOI: 10.1093/emboj/17.1.244.

L Lin et al. “The hepatocyte nuclear factor 3 α gene, HNF3α (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas”. Cancer Res 62(18) (2002), pp. 5273–5279.

LakminiK. B Mudduwa. “Quick score of hormone receptor status of breast carcinoma: Correlation with the other clinicopathological prognostic parameters”. Indian J Pathol Microbiol 52(2) (2009), pp. 159–159. DOI: 10.4103/0377-4929.48906.

Luka Bolha et al. “Comparison of methods for relative quantification of gene expression using real-time PCR”. Acta Agric Slov 100(2) (2012), pp. 97–106. DOI: 10.2478/v10014-012-0018-z.

M. A. Climent et al. “Prognostic value of HER-2/neu and p53 expression in node-positive breast cancer. HER-2/neu effect on adjuvant tamoxifen treatment”. Breast 10(1) (2001), pp. 67–77. DOI: 10.1054/brst.2000.0225.

Marjolein Droog et al. “Comparative Cistromics Reveals Genomic Cross-talk between FOXA1 and ERα in Tamoxifen-Associated Endometrial Carcinomas”. Cancer Res 76(13) (2016), pp. 3773–3784. DOI: 10.1158/0008-5472.can-14-1813.

Michael H Cho et al. “Folliculin mutations are not associated with severe COPD”. BMC Med Genet 9(1) (2008). DOI: 10.1186/1471-2350-9-120.

N A Alwan, F N Tawfeeq, and N A Mallah. “Demographic and clinical profiles of female patients diagnosed with breast cancer in Iraq”. J Contemp Med Sci 5(1) (2019), pp. 14–19.

Nidal M Almasri and Mohammad Al Hamad. “Immunohistochemical evaluation of human epidermal growth factor receptor 2 and estrogen and progesterone receptors in breast carcinoma in Jordan”. Breast Cancer Res 7(5) (2005), pp. 598–604. DOI: 10.1186/bcr1200.

Runnak A Majid et al. “Breast cancer in Iraq is associated with a unimodally distributed predominance of luminal type B over luminal type A surrogates from young to old age”. BMC Womens Health 17(1) (2017), pp. 27–28. DOI: 10.1186/s12905-017-0376-0.

Rutika J. Mehta et al. “FOXA1 is an independent prognostic marker for ER-positive breast cancer”. Breast Cancer Res Treat 131(3) (2012), pp. 881–890. DOI: 10.1007/s10549-011-1482-6.

S De Lara et al. “The prognostic relevance of FOXA1 and Nestin expression in breast cancer metastases: a retrospective study of 164 cases during a 10-year period”. BMC Cancer 19(1) (2004), pp. 187–187. DOI: 10.1186/s12885-019-5373-2.

Sunil Badve et al. “FOXA1 Expression in Breast Cancer—Correlation with Luminal Subtype A and Survival”. Clin Cancer Res 13(15) (2007), pp. 4415–4421. DOI: 10.1158/1078-0432.ccr-07-0122.

T. Sekiya et al. “Nucleosome-binding affinity as a primary determinant of the nuclear mobility of the pioneer transcription factor FoxA”. Genes Dev 23(7) (2009), pp. 804–809. DOI: 10.1101/gad.1775509.

W M T Al-Nuaimy, A H Ahmed, and H A A Al-Nuaimy. “Immunohistochemical Evaluation of Triple Markers (ER, PR and HER-2/neu) in Carcinoma of the Breast in the North of Iraq”. Don J Med Lab Diagn 1(1) (2015), pp. 1–009.

X C Wu et al. “Cancer in North America, 1995-1999”. Cancer in North America III(NAACCR Combined Cancer Incidence Rates) (2002).

X Dai et al. “FOXA1 is Prognostic of Triple Negative Breast Cancers by Transcriptionally Suppressing SOD2 and IL6”. Int J Biol Sci 15(5) (2019), pp. 1030–1041.

X Wang et al. “Functional Variant rs4442975 Modulating FOXA1 Binding Affinity Can Influence Bone Marrow Suppression during Neoadjuvant Chemotherapy for Luminal A Type Breast Cancer”. BioMed Res (2019).

Xiaoyong Fu et al. “FOXA1 overexpression mediates endocrine resistance by altering the ER transcriptome and IL-8 expression in ER-positive breast cancer”. Proc Natl Acad Sci U S A 113 (2016), E6600–E6609. DOI: 10.1073/pnas.1612835113.

Y A Yang and J Yu. “Current perspectives on FOXA1 regulation of androgen receptor signaling and prostate cancer”. Genes Dis 2 (2015), pp. 144–151. DOI: 10.1016/j.gendis.2015.01.003.

Yuichi Hisamatsu et al. “Impact of FOXA1 Expression on the Prognosis of Patients with Hormone Receptor-Positive Breast Cancer”. Ann Surg Oncol 19(4) (2012), pp. 1145–1152. DOI: 10.1245/s10434-011-2094-4.

Yuichi Hisamatsu et al. “Impact of GATA-3 and FOXA1 expression in patients with hormone receptor-positive/HER2-negative breast cancer”. Breast Cancer 22(5) (2015), pp. 520–528. DOI: 10.1007/s12282-013-0515-x.

Z Gu et al. “Clinical value of the changes of ER, PR, HER2 and Ki-67 expressions before and after neoadjuvant chemotherapy in breast cancer”. Open Sci J Clin Med 6(2) (2018), pp. 10–14.

Downloads

Published

2020-06-30

How to Cite

Al-Bedairy, I., Shamsa, M., Salim, S. aldeen, Mahdi, M., Dawood, K., & Al Faisal, A. H. (2020). FOXA1 expression in Iraqi women with ER+ breast cancer. Baghdad Journal of Biochemistry and Applied Biological Sciences, 2(02), 104–117. https://doi.org/10.47419/bjbabs.v2i02.43