FOXA1 expression in Iraqi women with ER+ breast cancer

Authors

  • Iman H. Al-Bedairy Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq
  • Mais S. Shamsa College of Medicine, University of Kufa, Kufa, Iraq
  • Safaa uldeen Salem College of Medicine, University of Kufa, Kufa, Iraq
  • Mohammed Mahdi DNA Forensic Center for Research and Training, Al-Nahrain University, Baghdad, Iraq
  • Karam Dawood Department of Medical Laboratory Technology, Al-Esraa University College, Baghdad, Iraq
  • Abdul Hussein M. Al Faisal Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.47419/bjbabs.v2i02.43

Keywords:

breast cancer, ER, FOXA1, immunohistochemistry, RT-qPCR

Abstract

Background: Breast cancer (BC) is a heterogeneous disease that can be classified into many subtypes according to histopathological and molecular characteristics. Forkhead box protein A1 (FOXA1) is a transcriptional pioneer factor that opens chromatin allowing estrogen receptor (α-ER) access to its genomic targets. FOXA1 expression is related to luminal BC with a good prognosis.

Objectives: The present study is sought to determine the FOXA1 expression in Iraqi women with ER+ BC.

Methods: Forty-eight fresh malignant breast tissues were analyzed by immunohistochemistry assay to choose ER+ samples, and then by RT-qPCR to evaluate FOXA1 gene expression.

Results: The ER-positive samples were (72.91%) of the total samples, and the molecular subtype of luminal A was the most common with a percentage of 56.25%. It was also noted that the high expression of the FOXA1 gene is highly significant (p<0.05) in Iraqi women with BC when compared with healthy controls.

Conclusions: Highly significant FOXA1 expression was found in Iraqi women with BC makes it eligible to be a good predictor or a biomarker for BC.

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Published

30-06-2020

How to Cite

Al-Bedairy, I., Shamsa, M., Salim, S. aldeen, Mahdi, M., Dawood, K., & Al Faisal, A. H. (2020). FOXA1 expression in Iraqi women with ER+ breast cancer. Baghdad Journal of Biochemistry and Applied Biological Sciences, 2(02), 106–119. https://doi.org/10.47419/bjbabs.v2i02.43