Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7
DOI:
https://doi.org/10.47419/bjbabs.v6i1.283Keywords:
Antimicrobial activity, Aronia melanocarpa, Extract, Lactic acid bacteria, Living cellAbstract
protecting and balancing the intestinal flora is important for protection body against many diseases. It is important to develop probiotics, which are an important part of the flora balance, and prebiotic agents that encourage the development of probiotics. The study aimed to determine the potential use of the ethanol extract obtained from Chokeberry fruits with probiotic candidate Limosilactobacillus fermentum MA-7 as natural additive agents in the pharmaceutical and food industries.The biological activity of the extract was determined against probiotic candidate lactic acid bacteria (LAB) strains isolated from human breast milk. First, the inhibitory activity of the extract was determined using the disc diffusion method against five different LABs. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the extract on the LABs were determined using the micro-dilution method. Finally, the biological activity of the extract on L. fermentum MA-7 at concentrations of 50 and 100 mg/ml was determined using the macro-dilution method.The extract showed antibacterial activity only against Streptococcus thermophilus MAS-1, with an inhibition zone diameter of 6.43 mm. MIC and MBC values of the extract were found as 12.5 mg/ml - >50 mg/ml and 25 mg/ml - >50 mg/ml, respectively. The stimulatory activity of the extract on L. fermentum MA-7 was obtained using macro-dilution method. The number of viable cells was determined after 0, 24 and 48 hours. A concentration of 50 mg/ml extract promoted the growth of L. fermentum MA-7 at 24 and 48 hours. 100 mg/ml extract concentration inhibited the growth of L. fermentum MA-7 after 24 hours but increased its growth after 48 hour compared to 24th hour. It has been shown that Chokeberry fruit ethanol extract at appropriate concentrations can be an alternative as a natural stimulant for L. fermentum MA-7 to support its development in the pharmaceutical industry and as a natural additive in the food industries.
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Ale EC, Rojas MF, Reinheimer JA, et al. Lactobacillus fermentum: Could EPS production ability be responsible for functional properties? Food Microbiology. 2020; 90:103465. https://doi.org/10.1016/j.fm.2020.103465 DOI: https://doi.org/10.1016/j.fm.2020.103465
Bintsis T. Lactic acid bacteria as starter cultures: An update in their metabolism and genetics. AIMS Microbiology. 2018;4(4):665. https://doi.org/10.3934%2Fmicrobiol.2018.4.665 DOI: https://doi.org/10.3934/microbiol.2018.4.665
Bontsidis C, Mallouchos A, Terpou A, et al. Microbiological and chemical properties of chokeberry juice fermented by novel lactic acid bacteria with potential probiotic properties during fermentation at 4 C for 4 weeks. Foods. 2021;10(4):768. https://doi.org/10.3390/foods10040768 DOI: https://doi.org/10.3390/foods10040768
Borgonovi TF, Virgolin LB, Janzantti NS, et al. Fruit bioactive compounds: Effect on lactic acid bacteria and on intestinal microbiota. Food Research International. 2022;161:111809. https://doi.org/10.1016/j.foodres.2022.111809 DOI: https://doi.org/10.1016/j.foodres.2022.111809
Borowska S, Brzóska MM. Chokeberries (Aronia melanocarpa) and their products as a possible means for the prevention and treatment of noncommunicable diseases and unfavorable health effects due to exposure to xenobiotics. Comprehensive Reviews in Food Science and Food Safety. 2016;15(6):982-1017. https://doi.org/10.1111/1541-4337.12221 DOI: https://doi.org/10.1111/1541-4337.12221
Buda V, Andor M, Diana A, et al. Cardioprotective Effects of Cultivated Black Chokeberries (Aronia spp.): Traditional Uses, Phytochemistry and Therapeutic Effects. In Bioactive Compounds in Nutraceutical and Functional Food for Good Human Health. IntechOpen. 2020;163-185. DOI: https://doi.org/10.5772/intechopen.92238
Chen H, Yan X, Du G, et al. Recent developments in antifungal lactic acid bacteria: application, screening methods, separation, purification of antifungal compounds and antifungal mechanisms. Critical Reviews in Food Science and Nutrition. 2023;63(15):2544-2558. https://doi.org/10.1080/10408398.2021.1977610 DOI: https://doi.org/10.1080/10408398.2021.1977610
Coman MM, Oancea AM, Verdenelli MC, et al. Polyphenol content and in vitro evaluation of antioxidant, antimicrobial and prebiotic properties of red fruit extracts. European Food Research and Technology. 2018;244:735-745. https://doi.org/10.1007/s00217-017-2997-9 DOI: https://doi.org/10.1007/s00217-017-2997-9
Imran Bashir KM, Lee JH, Petermann MJ, et al. Estimation of antibacterial properties of chlorophyta, rhodophyta and haptophyta microalgae species. Microbiology and Biotechnology Letters. 2018;46(3):225-233. http://dx.doi.org/10.4014/mbl.1802.02015 DOI: https://doi.org/10.4014/mbl.1802.02015
Jovandaric MZ, Dugalic S, Babic S, et al. Programming Factors of Neonatal Intestinal Dysbiosis as a Cause of Disease. International Journal of Molecular Sciences. 2023;24(6):5723. https://doi.org/10.3390/ijms24065723 DOI: https://doi.org/10.3390/ijms24065723
Jurendić T, Ščetar M. Aronia melanocarpa products and by-products for health and nutrition: A review. Antioxidants. 2021;10(7):1052. https://doi.org/10.3390/antiox10071052 DOI: https://doi.org/10.3390/antiox10071052
Jurikova T, Mlcek J, Skrovankova S, et al. Fruits of black chokeberry Aronia melanocarpa in the prevention of chronic diseases. Molecules. 2017;22(6):944. https://doi.org/10.3390/molecules22060944 DOI: https://doi.org/10.3390/molecules22060944
Kadıoğlu Y, Yılmaz Y. Samsun Örneğinde Klimatik Özelliklerin Aronya Yetiştiriciliği Açısından Analizi. Türk Tarım ve Doğa Bilimleri Dergisi. 2023;10(4):1137-1146. https://doi.org/10.30910/turkjans.1311377 DOI: https://doi.org/10.30910/turkjans.1311377
Kokotkiewicz A, Jaremicz Z, Luczkiewicz M. Aronia plants: a review of traditional use, biological activities, and perspectives for modern medicine. Journal of Medicinal Food. 2010;13(2):255-269. https://doi.org/10.1089/jmf.2009.0062 DOI: https://doi.org/10.1089/jmf.2009.0062
Kulling SE, Rawel HM. Chokeberry (Aronia melanocarpa)–A review on the characteristic components and potential health effects. Planta Medica. 2008;74(13):1625-1634. https://doi.org/10.1055/s-0028-1088306 DOI: https://doi.org/10.1055/s-0028-1088306
Kwa M, Plottel CS, Blaser MJ, et al. The intestinal microbiome and estrogen receptor–positive female breast cancer. Journal of the National Cancer Institute. 2016;108(8):djw029. https://doi.org/10.1093/jnci/djw029 DOI: https://doi.org/10.1093/jnci/djw029
Markowiak P, Śliżewska K. The role of probiotics, prebiotics and synbiotics in animal nutrition. Gut Pathogens. 2018;10(1):1-20. https://doi.org/10.1186/s13099-018-0250-0 DOI: https://doi.org/10.1186/s13099-018-0250-0
Meng L, Zhu J, Ma Y, et al. Composition and antioxidant activity of anthocyanins from Aronia melanocarpa cultivated in Haicheng, Liaoning, China. Food Bioscience. 2019;30:100413. https://doi.org/10.1016/j.fbio.2019.100413 DOI: https://doi.org/10.1016/j.fbio.2019.100413
Nanasombat S, Kuncharoen N, Ritcharoon B, et al. Antibacterial activity of thai medicinal plant extracts against oral and gastrointestinal pathogenic bacteria and prebiotic effect on the growth of Lactobacillus acidophilus. Chiang Mai Journal of Science. 2018;45(1):33-44.
Raman J, Kim JS, Choi KR, et al. Application of lactic acid bacteria (LAB) in sustainable agriculture: Advantages and limitations. International Journal of Molecular Sciences. 2022;23(14):7784. https://doi.org/10.3390/ijms23147784 DOI: https://doi.org/10.3390/ijms23147784
Staudacher HM, Lomer MC, Farquharson FM, et al. A diet low in FODMAPs reduces symptoms in patients with irritable bowel syndrome and a probiotic restores Bifidobacterium species: a randomized controlled trial. Gastroenterology. 2017;153(4):936-947. https://doi.org/10.1053/j.gastro.2017.06.010 DOI: https://doi.org/10.1053/j.gastro.2017.06.010
Sutherland J, Miles M, Hedderley D, et al. In vitro effects of food extracts on selected probiotic and pathogenic bacteria. International Journal of Food Sciences and Nutrition. 2009;60(8):717-727. https://doi.org/10.3109/09637480802165650 DOI: https://doi.org/10.3109/09637480802165650
Szopa A, Kokotkiewicz A, Kubica P, et al. Comparative analysis of different groups of phenolic compounds in fruit and leaf extracts of Aronia sp.: A. melanocarpa, A. arbutifolia, and A. prunifolia and their antioxidant activities. European Food Research and Technology. 2017;243: 1645-1657. https://doi.org/10.1007/s00217-017-2872-8 DOI: https://doi.org/10.1007/s00217-017-2872-8
Şahin A, Erdoğan Ü. Aronia (Aronia melanocarpa Michx Elliot) Production and Evaluation Methods in the World and Turkey. Turkish Journal of Agriculture-Food Science and Technology. 2022;10(1):81-85.https://doi.org/10.24925/turjaf.v10i1.81-85.4547 DOI: https://doi.org/10.24925/turjaf.v10i1.81-85.4547
Tarique M, Abdalla A, Masad R, et al. Potential probiotics and postbiotic characteristics including immunomodulatory effects of lactic acid bacteria isolated from traditional yogurt-like products. Learning with Technologies. 2022; 159:113207. https://doi.org/10.1016/j.lwt.2022.113207 DOI: https://doi.org/10.1016/j.lwt.2022.113207
Tamkutė L, Vaicekauskaitė R, Gil BM, et al. Black chokeberry (Aronia melanocarpa L.) pomace extracts inhibit food pathogenic and spoilage bacteria and increase the microbiological safety of pork products. Journal of Food Processing and Preservation. 2021;45(3):e15220. https://doi.org/10.1111/jfpp.15220 DOI: https://doi.org/10.1111/jfpp.15220
Xiao L, Feng Q, Liang S, et al. A catalog of the mouse gut metagenome. Nature Biotechnology. 2015;33(10):1103-1108. https://doi.org/10.1038/nbt.3353 DOI: https://doi.org/10.1038/nbt.3353

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